Evaluation of malondialdehyde and glutathione peroxidase changes in iron deficiency anemia patients and their correlation with body iron status and haematological indices

Abstract

Iron deficiency anemia (IDA) is a prevalent disorder among women of reproductive age. This study aims atinvestigating whether the activities of glutathione peroxidase (GPx) and malondialdehyde (MDA) levels canbe affected during iron deficiency anemia among women of reproductive age. In this study, 30 women withIDA, 30 with iron deficiency and 30 healthy controls were studied. The concentrations of serum malondialdehydewere measured and the activities of GPx were evaluated in all the three groups. The mean serumconcentration of MDA was significantly higher in IDA group than ID group and healthy group women(5.180.46 vs 3.40.34 and 2.750.33 ?mol/L) respectively. The mean GPx activity in IDA groups wassignificantly lower than healthy group women (1.40.2 vs 2.910.12 and 2.990.24 (U/gHb)). The concentrationof MDA in IDA and ID group was negatively correlated with levels of haemoglobin (Hb), meancorpuscular volume (MCV) and serum ferritin (p?0.05). In healthy control subjects; there was a negativecorrelation between MDA concentration and Hb, MCV and serum ferritin (p?0.01). Furthermore, the activityof GPx and these factors levels showed significant positive correlation in every group (p ?0.01 and p?0.05). Our findings showed that lipid peroxidation increases in women with ID and IDA but the activity ofGPx decreases in IDA but not in ID. Moreover, there was no significant correlation between serum freeiron levels with MDA concentration and GPx activity.     Iron deficiency anemia (IDA) is a prevalent disorder among women of reproductive age. This study aims atinvestigating whether the activities of glutathione peroxidase (GPx) and malondialdehyde (MDA) levels canbe affected during iron deficiency anemia among women of reproductive age. In this study, 30 women withIDA, 30 with iron deficiency and 30 healthy controls were studied. The concentrations of serum malondialdehydewere measured and the activities of GPx were evaluated in all the three groups. The mean serumconcentration of MDA was significantly higher in IDA group than ID group and healthy group women(5.180.46 vs 3.40.34 and 2.750.33 ?mol/L) respectively. The mean GPx activity in IDA groups wassignificantly lower than healthy group women (1.40.2 vs 2.910.12 and 2.990.24 (U/gHb)). The concentrationof MDA in IDA and ID group was negatively correlated with levels of haemoglobin (Hb), meancorpuscular volume (MCV) and serum ferritin (p?0.05). In healthy control subjects; there was a negativecorrelation between MDA concentration and Hb, MCV and serum ferritin (p?0.01). Furthermore, the activityof GPx and these factors levels showed significant positive correlation in every group (p ?0.01 and p?0.05). Our findings showed that lipid peroxidation increases in women with ID and IDA but the activity ofGPx decreases in IDA but not in ID. Moreover, there was no significant correlation between serum freeiron levels with MDA concentration and GPx activity.